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Image Search Results
Journal: Frontiers in Immunology
Article Title: TGF-β and TNF-α interaction promotes the expression of MMP-9 through H3K36 dimethylation: implications in breast cancer metastasis
doi: 10.3389/fimmu.2024.1430187
Figure Lengend Snippet: MMP-9 expression is associated with TGF-β and TNF-α in breast cancer tissues. Immunohistochemical staining was done for MMP-9, TGF-β, and TNF-α expressions. Tissue microarray TMA slides (88 breast cancer tissues from breast cancer patients) were subjected to immunohistochemistry using antibodies against MMP-9, TGF-β, and TNF-α. Based on IHC staining intensities of TGF-β and TNF-α, patients’ samples were divided into four groups (Low TNF-α- Low TGF-β; Low TNF-α- High TGF-β; High TNF-α- Low TGF-β; High TNF-α- High TGF-β. (A) Scatter Plot Graph for TNF-α and TGF-β. (B) Scatter Plot Graph for TNF-α and TGF-β with indication of patients with primary cancer, metastasis and normal breast cancer tissues. (C) MMP-9 expression was elevated in the breast cancer tissues (n=24 patients) having high levels of TGF-β and TNF-α compared to the tissues (n=20 patients) having less TGF-β and TNF-α. (D) Representative IHC images of the samples of breast cancer tissues with low levels of MMP-9, TGF-β and TNF-α. (E) Representative IHC images of the samples of breast cancer tissues showing high levels of MMP-9, TGF-β and TNF-α. All data is presented as the mean ± SD. *p < 0.05.
Article Snippet: Ethical approval was not required for the studies involving humans because We purchased commercial
Techniques: Expressing, Immunohistochemical staining, Staining, Microarray, Immunohistochemistry
Journal: Cancer Medicine
Article Title: Upregulated IQUB promotes cell proliferation and migration via activating Akt/ GSK 3β/β‐catenin signaling pathway in breast cancer
doi: 10.1002/cam4.1568
Figure Lengend Snippet: IQUB is significantly upregulated in human breast cancer tissues and cells. A, IQUB protein expression (the brown staining areas) was increased in breast cancer, which was detected in 110 cases of human breast cancer tissue microarray by immunohistochemistry. B, The expression of IQUB in poor differentiation of breast cancer tissues was higher than that in well differentiation of breast cancer tissues. C, IQUB mRNA expression was upregulated in breast cancer tissues (16/20) than paired normal breast tissues which was analyzed by RT ‐ qPCR ( x ‐axis represent 20 pairs of tissue samples). D, IQUB mRNA and protein expression were detected by RT ‐ qPCR and Western blotting in normal breast cell line MCF ‐10A and breast cancer cell lines MCF ‐7 and MDA ‐ MB ‐231. Values represent the mean ± SD from 3 independent measurements. ** P < .01, *** P < .001
Article Snippet: The
Techniques: Expressing, Staining, Microarray, Immunohistochemistry, Quantitative RT-PCR, Western Blot